Machado Hidevaldo B, Vician Linda J, Herschman Harvey R The MAPK pathway is required for depolarization-induced .
Journal of neuroscience research.
2008;
86(3):
593-602.
Weber Wolfgang A, Czernin Johannes, Phelps Michael E, Herschman Harvey R Technology Insight: novel imaging of molecular targets is an emerging area crucial to the development of targeted drugs..
Nature clinical practice. Oncology.
2008;
5(1):
44-54.
Ishikawa Tomo-o, Herschman Harvey R Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome..
Journal of cellular biochemistry.
2007;
102(6):
1486-92.
Sui Guodong, Lee Cheng-Chung, Kamei Ken-Ichiro, Li Hua-Jung, Wang Jin-Yi, Wang Jun, Herschman Harvey R, Tseng Hsian-Rong A microfluidic platform for sequential ligand labeling and cell binding analysis..
Biomedical microdevices.
2007;
9(3):
301-5.
Li Hua-Jung, Everts Maaike, Pereboeva Larisa, Komarova Svetlana, Idan Anat, Curiel David T, Herschman Harvey R Adenovirus tumor targeting and hepatic untargeting by a coxsackie/adenovirus receptor ectodomain anti-carcinoembryonic antigen bispecific adapter..
Cancer research.
2007;
67(11):
5354-61.
Koya Richard C, Kimura Takahiro, Ribas Antoni, Rozengurt Nora, Lawson Gregory W, Faure-Kumar Emmanuelle, Wang He-jing, Herschman Harvey, Kasahara Noriyuki, Stripecke Renata Lentiviral vector-mediated autonomous differentiation of mouse bone marrow cells into immunologically potent dendritic cell vaccines..
Molecular therapy : the journal of the American Society of Gene Therapy.
2007;
15(5):
971-80.
Xu Zheng, Choudhary Shilpa, Voznesensky Olga, Mehrotra Meenal, Woodard Monica, Hansen Marc, Herschman Harvey, Pilbeam Carol Overexpression of COX-2 in human osteosarcoma cells decreases proliferation and increases apoptosis..
Cancer research.
2006;
66(13):
6657-64.
Kamei Ken-ichiro, Ishikawa Tomo-o, Herschman Harvey R Transgenic mouse for conditional, tissue-specific Cox-2 overexpression..
Genesis (New York, N.Y. : 2000).
2006;
44(4):
177-82.
Ishikawa Tomo-O, Herschman Harvey R Conditional knockout mouse for tissue-specific disruption of the cyclooxygenase-2 (Cox-2) gene..
Genesis (New York, N.Y. : 2000).
2006;
44(3):
143-9.
Czernin Johannes, Weber Wolfgang A, Herschman Harvey R Molecular imaging in the development of cancer therapeutics..
Annual review of medicine.
2006;
57(3):
99-118.
Lu Gang, Kang Young Jun, Han Jiahuai, Herschman Harvey R, Stefani Enrico, Wang Yibin TAB-1 modulates intracellular localization of p38 MAP kinase and downstream signaling..
The Journal of biological chemistry.
2006;
281(9):
6087-95.
Chikazu Daichi, Li Xiaodong, Kawaguchi Hiroshi, Sakuma Yoko, Voznesensky Olga S, Adams Douglas J, Xu Manshan, Hoshi Kazuto, Katavic Vedran, Herschman Harvey R, Raisz Lawrence G, Pilbeam Carol C Bone morphogenetic protein 2 induces cyclo-oxygenase 2 in osteoblasts via a Cbfa1 binding site: role in effects of bone morphogenetic protein 2 in vitro and in vivo. 2002..
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.
2005;
20(10):
1888-98.
Yaghoubi Shahriar S, Barrio Jorge R, Namavari Mohammad, Satyamurthy Nagichettiar, Phelps Michael E, Herschman Harvey R, Gambhir Sanjiv S Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography..
Cancer gene therapy.
2005;
12(3):
329-39.
Vician Linda J, Xu Guoping, Liu Wei, Feldman Jonathan D, Machado Hidevaldo B, Herschman Harvey R MAPKAP kinase-2 is a primary response gene induced by depolarization in PC12 cells and in brain..
Journal of neuroscience research.
2004;
78(3):
315-28.
Singh Vinita, Miranda Tina Branscombe, Jiang Wei, Frankel Adam, Roemer Martha E, Robb Victoria A, Gutmann David H, Herschman Harvey R, Clarke Steven, Newsham Irene F DAL-1/4.1B tumor suppressor interacts with protein arginine N-methyltransferase 3 (PRMT3) and inhibits its ability to methylate substrates in vitro and in vivo..
Oncogene.
2004;
23(47):
7761-71.
Ferguson Gregory D, Herschman Harvey R, Storm Daniel R Reduced anxiety and depression-like behavior in synaptotagmin IV (-/-) mice..
Neuropharmacology.
2004;
47(4):
604-11.
Machado Hidevaldo B, Liu Wei, Vician Linda J, Herschman Harvey R Synaptotagmin IV overexpression inhibits depolarization-induced exocytosis in PC12 cells..
Journal of neuroscience research.
2004;
76(3):
334-41.
Ferguson Gregory D, Wang Hongbing, Herschman Harvey R, Storm Daniel R Altered hippocampal short-term plasticity and associative memory in synaptotagmin IV (-/-) mice..
Hippocampus.
2004;
14(8):
964-74.
Chen Ian Y, Wu Joseph C, Min Jung-Jun, Sundaresan Gobalakrishnan, Lewis Xiaoman, Liang Qianwa, Herschman Harvey R, Gambhir Sanjiv S Micro-positron emission tomography imaging of cardiac gene expression in rats using bicistronic adenoviral vector-mediated gene delivery..
Circulation.
2004;
109(11):
1415-20.
Herschman Harvey R Noninvasive imaging of reporter gene expression in living subjects..
Advances in cancer research.
2004;
92(2):
29-80.
Symensma Tonia L, Martinez-Guzman DeeAnn, Jia Qingmei, Bortz Eric, Wu Ting-Ting, Rudra-Ganguly Nandini, Cole Steve, Herschman Harvey, Sun Ren COX-2 induction during murine gammaherpesvirus 68 infection leads to enhancement of viral gene expression..
Journal of virology.
2003;
77(23):
12753-63.
Herschman Harvey R Molecular imaging: looking at problems, seeing solutions..
Science (New York, N.Y.).
2003;
302(5645):
605-8.
Liu Wei, Feldman Jonathan D, Machado Hidevaldo B, Vician Linda J, Herschman Harvey R Expression of depolarization-induced immediate early gene proteins in PC12 cells..
Journal of neuroscience research.
2003;
72(6):
670-8.
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Identification and Characterization of Genes that Modulate Mitogenesis and Inflammatory Responses. We study how extracellular stimuli elicit changes in cell growth and differentiation, as a result of induced transcription of quiescent primary response genes. We cloned seven "TIS" genes whose transcription is rapidly induced by mitogens in growth-arrested fibroblasts. The TIS genes are all induced by activation of pre-existing transcription factors. The TIS10 gene, now called COX-2, encodes a second form of cyclooxygenase (COX), the rate-limiting enzyme responsible for production of prostaglandins in acute inflammation. COX-2 is the molecular target for Vioxx and Celebrex, the largest selling group of compounds ever to enter the pharmaceutical industry. We study (i) cis-acting promoter sequences and transacting factors regulating COX-2 expression in response to growth factors, inflammatory agents, and cytokines and (ii) the biological role of COX-2 in ligand-induced proliferation,inflammation and cancer.
Identification and Charactizattion of Genes Regulating Neuronal Differentiation and Function: We have identified genes that are expressed in neurons either (i) in a neuron-specific manner, following depolarization, or (ii) in neuronal precursor cells in response to neurotrophins such as nerve growth factor. We identified synaptotagmin IV as a gene induced by depolarization in hippocampal neurons, but not by growth factors, hormones, etc. Syt IV, like other synaptotagmins, is found in synaptic vesicles after its induction. Synaptotagmins mediate stimulus-initiated, calcium-dependent fusion of synaptic vesicles to presynaptic membrane. Syt4 appears to be a molecule which, as a result of neuronal stimulation, alters the composition of the synaptic machinery. Syt IV knockout mice, created in our laboratory, are viable but show defects in learning hippocampal dependent tasks. We are now studying the regulation of Syt IV expression by depolarization, the biochemical properties of Syt IV, and the role of Syt IV in memory and learning. We have isolated a number of additional depolarization-induced genes whose roles in memory and learning we will also study.
Non-invasive, Repetitive and Quantitatitve Imaging of Reporter Gene Expression in Living Animals. The use of reporter genes such as luciferase, beta-galactosidase and green flourescent protein has opened new areas in cell biology. We have recently developed reporter gene systems that can be used to image gene expression in living vertebrates. We use reporter genes whose protein products can trap positron labeled substrates in tissues, and then image the reporter gene dependent trapping of the positron labeled reporter probes with by positron emission tomography. We have developed two PET reporter gene systems; (1) an enzyme based system that uses the Herpes Simplex Virus thmidine kinase as the reporter gene and positron labelled substrates as a reporter probe and (2) a receptor based system that uses the Dopamine D2 Receptor as the reporter gene and positron labeled ligand as the reporter probe. More recently, we have begun to use optical reporter systems that allow us to monitor gene expression with luciferase in living animals with sensitive CCD cameras. We are currently applying these techniques to transgenic mouse models of disease and to development of gene therapy vectors whose location and expression can be monitored in living subjects.
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